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From the filtrate, 5 μL was spotted onto the plate and then development, staining and scanning were done as previously described.
ChIP-chip hybridization and scanning were done by Nimblegen.
Labeling, hybridization and scanning were done as previously described [6].
Hybridization, washing, and scanning were done according to standard Affymetrix protocols.
Complementary DNA synthesis, cRNA synthesis, hybridization, washing, and scanning were done following the manufacturer's protocols (Affymetrix, Inc).
Complementary DNA synthesis, cRNA synthesis, hybridization, washing, and scanning were done following the manufacturer's protocols (Affymetrix, Inc .. Expression values were calculated after normalization of the data based on previously published procedure [32].
Similar(39)
All of the scanning was done at the Athinoula A. Martinos Imaging Center at the McGovern Institute.
Asher said the scanning was done at the discretion of officers at the gates.
Scanning was done at 28 μm isotropic voxel size to produce five image stacks of each femur.
UV scanning was done at 370 nm.
Transportation, deployment, calibration and the actual scanning was done in around 6 hours.
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