Vehicle or acetaminophen at a dose of 100 or 300 mg/kg was intraperitoneally administered, and PET scanning was conducted using 18F-BCPP-BF 2 or 24 h after the administration of acetaminophen.
Scanning was conducted using a GeneChip Scanner 3000.
Scanning was conducted using an Axon Genepix 4200A scanner with Genepix Pro 6.1 image acquisition software (Molecular Devices, UK) with 60%% red and 90%% green laser power at 5 μm resolution.
All AFM scans were conducted using an Innova scanning probe microscope (Veeco, U.S.A .. Scans were performed in tapping mode at ambient temperature and pressure, using silicon cantilevers (MPP-31120-10, Veeco, U.S.A).
For the estimation of HOMO and LUMO energy levels, cyclic voltammetry (CV) scans were conducted using a Gamry 600 reference potentiostat (Gamry Instruments, Inc., Warminster, USA) equipped with virtual front panel software.
After an intravenous injection of 11C-PBB3 (injectedosese, 399 ± 45 MBq; specific activity, 88 ± 32 GBq/μmol), 70-min dynamic PET scans were conducted using an ECAT Exact HR+ system (developed by CTI, Knoxville, TN, USA, and distributed by Siemens Healthcare, Erlangen, Germany).
All BMD scans were conducted using standardized procedures following the manufacturer's recommended protocols by well-trained examiners.
All PET scans were conducted using an ECAT HR+ 962 scanner (CTI/Siemens, Knoxville, TN, USA), with an axial field of view of 15.5 cm.
For functional localization of V5, four functional scans were conducted using a T2*-weighted gradient echo, EPI sequence (TR = 2 s, TE = 30 ms, and flip angle = 65°) to acquire 160 volumes constructed from 39 axial slices covering the whole brain at voxel resolution of 3 × 3 × 3 mm.
A genome scan was conducted using the same methods as above.
