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Quality tests, dye-binding, hybridization and microarray scanning were conducted by Oaklabs.
DNA amplification, fragmentation, chip hybridization, single base extension, staining and scanning were conducted by the Life Science and Technology Center, China National Seed Group Co., LTD (Wuhan, China).
DNA amplification, fragmentation, chip hybridization, single base extension, staining and scanning were conducted by Life Science and Technology Center, China National Seed Group Co., LTD (Wuhan, China), according to Infinium HD Assay Ultra Protocol (http://www.illumina.com/).
All processes for cDNA and cRNA synthesis, cRNA fragmentation, hybridization, washing and staining, and scanning, were conducted according to the GeneChip Standard Protocol (Eukaryotic Target Preparation, Affymetrix).
Probe labeling, hybridization and scanning were conducted according to the manufacturer's instructions (Affymetrix, http://www.affymetrix.com).
Microarray hybridizations, washing, and scanning were conducted as described previously [ 5].
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Scanning was conducted at the Center for Advanced Imaging, University of Queensland (Inveon multimodel Siemens PET/CT scanner) or The University of New England (Vtomexs system GE Phoenix scanner).
To gather the NDVI data at different crop growth stage, field scanning was conducted for many times during my intern.
PET scanning was conducted as described in the legend of Fig. 1.
Scanning was conducted under 2.5% isoflurane gas anesthesia using the MicroPET Focus120 small animal scanner (Concorde/Siemens, Knoxville, TN, USA) [25].
Scanning was conducted prior to examining or analyzing questionnaires scores.
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