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Serial blood samples before and after scanning were collected for measurement of plasma glucose, insulin, gut hormones (PYY, GLP-1 and acyl ghrelin) and bile acids (see online supplementary figure S1).

A total of 256 scans were collected for each sample.

About 10,000 scans were collected for samples (Quideau et al. 2001).

Multiple high-resolution T1- weighted volumetric MP-RAGE scans were collected for each subject and the raw DICOM images were downloaded from the public ADNI site (http://www.loni.ucla.edu/ADNI/Data/index.shtml).shtml

The emission scans were collected for each complex separately and the change in maximum fluorescence signal at 335 nm (due to tyrosinate ions) was used to generate the binding curves, after subtracting the contribution of the free protein (in the absence of any compounds).

Twenty scans were collected for every acquisition.

Typically, 64 4.28-s scans were collected for each sample with a pre-saturation pulse to suppress the water signal.

Three scans were collected for each protein sample and baseline (which consisted of the column flow-through following protein concentration).

One thousand scans were collected for each sample and buffer spectrum from 2200 to 1950 cm 1 at a nominal resolution of 2 cm–1.

For ex situ measurements after incubation, 64 scans were co-averaged while 128 scans were collected for each time frame in situ experiments during incubation.

The recycle delay was set to 1.5 s and 128 scans were collected for each increment for a total experiment time of 14.5 h.