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Microarray probe preparation, hybridization on Agilent Whole Genome 44k oligo microarrays and scanning were carried out as previously described [27].
For Affymetrix Microarray analysis, fragmentation of RNA, labeling, hybridization to HG-U133 Plus 2.0 microarrays, and scanning were carried out according to the manufacturer's protocol (Affymetrix Inc).
Microarray hybridization, washing and scanning were carried out on an Affymetrix Fluidics Station 400 and an Agilent GeneArray Scanner according to standard protocols provided by Affymetrix , Inc
cDNA production and labeling, hybridization and array scanning were carried out by MOgene (St . Louis MO), using the DNase I-digested RNA.
In vitro transcription (IVT) products were generated and oligonucleotide array hybridization and scanning were carried out according to the instructions supplied by Affymetrix (Santa Clara, CA).
RNA quality check, target labeling, GeneChip hybridization, and oligonucleotide microarray scanning were carried out at Seqwright (Houston, TX, USA) on the GeneChip Drosophila 2.0 Array (Affymetrix, Santa Clara, CA) following a standard Affymetrix protocol.
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Scanning was carried out in air and at a scanning rate of 10 Hz.
Scanning is carried out over the range from 2Ө = 4° to 80°.
Scanning was carried out at a speed of 0.04 deg/s with a measurement range of 1° 70°.
Finally, scanning was carried out using CAMAG TLC densitometric Scanner 3S/N 130319 with WINCATS software (CAMAG, Muttens, Switzerland).
MA-XRF scanning was carried out with the M6 Jetstream from Bruker AXS.
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