Vehicle or acetaminophen at a dose of 100 or 300 mg/kg was intraperitoneally administered, and PET scanning was conducted using 18F-BCPP-BF 2 or 24 h after the administration of acetaminophen.
Scanning was conducted using a GeneChip Scanner 3000.
Scanning was conducted using an Axon Genepix 4200A scanner with Genepix Pro 6.1 image acquisition software (Molecular Devices, UK) with 60%% red and 90%% green laser power at 5 μm resolution.
Hybridisation, washing, staining and scanning were conducted using the same instruments for all samples.
A genome scan was conducted using the same methods as above.
The scan was conducted using HMMSearch from profile HMM package HMMER, obtained from Washington University [ 38].
In this study, a genome wide scan was conducted using bulks of at least 20 F2 individuals, and mapping intervals of several cM were achieved.
The genome scan was conducted using an Illumina Mouse Medium density SNP panel (Genetic Analysis Facility, Centre for Applied Genomics, Hospital for Sick Children, Toronto, ON).
All AFM scans were conducted using an Innova scanning probe microscope (Veeco, U.S.A .. Scans were performed in tapping mode at ambient temperature and pressure, using silicon cantilevers (MPP-31120-10, Veeco, U.S.A).
For the estimation of HOMO and LUMO energy levels, cyclic voltammetry (CV) scans were conducted using a Gamry 600 reference potentiostat (Gamry Instruments, Inc., Warminster, USA) equipped with virtual front panel software.
After an intravenous injection of 11C-PBB3 (injectedosese, 399 ± 45 MBq; specific activity, 88 ± 32 GBq/μmol), 70-min dynamic PET scans were conducted using an ECAT Exact HR+ system (developed by CTI, Knoxville, TN, USA, and distributed by Siemens Healthcare, Erlangen, Germany).
