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After micro-CT scanning, specimens were sterilized (as described above) prior to being immersed in fresh liquid and kept at 37°C until the next time point.
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The predicted fatigue life and crack initiation position for CT scanned specimens were compared with experimental results.
The scanned specimens were whole sub-adult or adult individuals ranging in diameter or length from 5 43 mm.
The μCT data of the scanned specimen was imported to the software Avizo 7.0 (FEI-VSG company), where a reconstruction and segmentation were performed, and bone, teeth and matrix were separated in layers to analyse inner bone structures.
Prior to scanning, the specimens were taken out of their jars, thoroughly rinsed with demineralised water and placed in disposable plastic bags to prevent dehydration during imaging.
After the first μCT scan, the specimens were taken out from the scan cabin and joint capsule was incised to separate the femoral head from acetabulum.
For scanning electron microscopy, specimens were dehydrated through immersion in a standard ethanol series, impregnated for 24 hours in hexamethyldisilazane, air dried and gold coated.
For μCT scan, long bone specimens were rehydrated with PBS and μCT scan was performed.
Pigs were followed by CT scan and explanted specimens were analyzed for bone tissue remodeling by micro-CT scan, micro-radiography, and histology/histomorphometry.
The procedure of scanning the specimens is described as well as the tests performed using the proposed algorithm.
Computer tomographic scanning of specimens was performed to evaluate stem alignment after surgery.
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