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To improve the reliability of quantitative analysis, 3D optical profilometry was used to determine the Ra values by scanning sample areas rather than performing in-line analysis on enamel areas [23].
Fluorescence readings were taken by exciting samples at 440 nm and emission intensities were obtained by scanning sample emissions from 460 nm–620 nm using a fluorometer (Perkin Elmer, LS50B).
For the scanning sample, 27% of selected 80S particles (171,143) were computationally classified as being in an unratcheted conformation and bound to a P-site tRNA.
All models were initially built and refined in the density for the higher resolution engaged complex, and then rigid body fit into the density for the scanning sample.
It is worth noting that while the scanning sample also contains ∼40% of particles bound to eEF2, no density was observed surrounding the exit tunnel.
Light from the laser was split into a single pass reference arm and a pre-objective scanning sample arm where the galvanometric scanners are placed in the back focal plane of the objective lenses.
Similar(53)
In order to study the thermal conduction mechanism, we obtained gray images of coal mass microstructure by scanning samples with a digital microscope.
Fourth, after scanning, samples are run through the programme to test if quantification is reliable.
Following micro-CT scanning, samples were embedded in polymethylmethacrylate (PMMA).
Prior to scanning, samples were degassed at 50 mbar.
During scanning, samples were enclosed in tightly fitting plastic wrap to prevent movement and dehydration.
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