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The first scan of the transcript yielded 19 possible categories of teaching moments and after 5 complete iterations of the 53-page transcript, six categories remained (Table 1), with no further changes on the final iteration.The distribution of the different types of teaching used is significantly different.
Consequently, RT-PCR scanning of the patient transcript was unable to generate amplicons in the region of the mutation, when tens of kilobases of intronic sequence must also be amplified.
For each miRNA, we scanned the 3′UTRs of the transcript set for all sites complementary to a miRNA subsequence beginning at either position one (α-position), two (β-position) or three (γ-position) relative to the miRNA 5′-end.
One such mechanism is "leaky scanning" where a proportion of the scanning complexes bypass the uORF AUG and continue scanning the transcript onto a downstream start codon.
Save a scanned copy of the updated transcript and e-mail it as a PDF attachment to [email protected]
For each category, we scanned the transcript for the presence of a UCCC motif as described previously [26].
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The resulting enriched transcript is time-aligned to the original speech, allowing users to scan the transcript as a whole or the additional visual cues present in the transcript, to fixate and play regions of interest.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com