Activated galectin-3 mAb (400 µg /200 µl volume) was labeled by adding 7 µl of a methylen-diposphonate (MDP) bone scan kit (Medrotec®, Amersham Health, UK) as previously reported [22] and 10 mCi of 99mTcO4− freshly eluted from (99Mo/99mTc) generator (GE-Healthcare, UK).
We evaluated the phosphorylation levels of key transcription factors in tOASFs using a commercial pathway scan kit.
Using a commercial pathway scan kit and western blotting (Supplementary Figure S2), the activation levels of inflammatory-associated key intracellular signaling proteins in tOASFs were evaluated.
In contrast, Surveyor Scan Kits, BEAMing technology and pyrosequencing were used in pivotal studies, and RAS mutations were detected with 1 10% sensitivity [ 12- 18].
Of 597 patients with KRAS exon 2 WT tumours and RAS data, 107 (18 %) had other RAS mutations (RAS status determined using bidirectional Sanger sequencing and WAVE-based Surveyor® Scan Kits (Transgenomic) cIncluded only patients with baseline measurable disease per central review Results of the final analysis were consistent with the primary analysis.
Previously, we analyzed the 1-kb espB gene of EPEC using the linker scanning mutagenesis kit from New England Biolabs, which is based on in vitro Tn 7 random insertions (4).
Relative expression levels of PTPN4 in different tissues of wild-type mice were determined by quantitative PCR of cDNA samples from a Tissue Scan RT kit (Origene).
Gels were stained with a silver staining kit (Amersham Biosciences) and scanned by using a UMAX power look 1120 scanner (UMAX, Taiwan).
The fragmented-Biotin-labeled cRNA was then added to the array (Affymetrix GeneChip miRNA 3.0), and after washing to remove any unbound RNA, hybridization was assessed by fluorescent staining (GeneChip Hybridization, Wash and Stain kit) and scanning with a GeneChip Scanner 3000 system.
The arrays were washed using the NimbleGen Wash kit and scanned using the MS 200 scanner (Roche NimbleGen) in two channels: 532 nm and 635 nm.
Blots and the developed control (k) strip, supplied with each kit, were scanned and magnified, to maximize precision of band detection.
