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Laser scanning imaging was used to measure local cerebral blood flow (LCBF) as previously described (Li et al., 2007) at three time points: immediately before MCA ligation, right after occlusion, during the 7 min bilateral CCA ligation and 21 days after ischemia.
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CT scan imaging was performed at the same intervals to follow the evolution of the disease.
Line-scan imaging was performed to record Ca2+ signals in the dendritic shaft and one to four spines.
CT scan imaging was not used as the evaluation measure, as four patients still had small lamellar high density lesion on CT imaging.
Confocal line-scan imaging was performed using a Leica SP5 confocal microscope (Germany, 40 ×, 1.25 NA) with an excitation at 488 nm.
Side-scan imaging is a technology that can be used to survey wide areas and that can produce images of shape and dimensions (Fig. 1c).
Line-scan imaging is synchronized with stage position so that variations in imaging speed do not affect resolution or image quality.
In this work, the THz scanning transmission imaging was successfully demonstrated for measuring the freshness of a vegetable leaf over a period of time.
Laser scanning confocal imaging was done with a Zeiss LSM 510 confocal microscope.
Scanning electron microscopy imaging was performed for pore size measurement and cell attachment.
Scanning electron microscopy imaging was done with SEM LEO 1550 Gemini.
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