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To examine the interaction between GH GPI and GHR, we monitored the localization of the fluorescent fusion proteins by laser scanning fluorescence microscopy.
Fluorescent signal intensity was measured via scanning fluorescence spectrometry using SPEX Fluorolog II spectrophotometer (Horiba Jobin Yvon Inc., NJ, USA).
Figure 9 Confocal laser scanning fluorescence microscopyimages of cells labeled with CHPNH 2 (15 -QD nanoparticle.
Fig. 10 Line scanning fluorescence image of PC-based nanofluidic sensor: on resonance and off resonance.
Absence of cytotoxicity in 208F fibroblasts correlated with poor intracellular peptide uptake, as monitored by confocal laser scanning fluorescence microscopy.
The results demonstrated that they were non-toxic and effective fluorophores for bioimaging live HEK293T cells (human embryonic kidney cells) using confocal laser scanning fluorescence microscopy.
The cooperative effects of multi-branched structures towards metal ions were carried out by UV vis absorption titrations and time scanning fluorescence spectroscopic.
Confocal laser scanning fluorescence microscopy (Zeiss LSM Meta 510, Carl Zeiss, Germany) was used to detect the fluorescence of the cells using a 488-nm argon laser irradiation to excite the QD chitosan nanoconjugates.
Fluorescence detection was performed on 10 μL of the bacterial suspension that was placed on a glass slide and observed under Leica scanning fluorescence microscope (Leica Microsystems, Buffalo Grove, IL, USA)[3].
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The images were captured using a confocal laser-scanning fluorescence microscope Leica SP5 (Molecular Probes, Leica Microsystem) at 63× magnification.
The cells were examined for immunostaining signals with a confocal laser-scanning fluorescence microscope (Fluoview FV1000-D; Olympus, Tokyo, Japan).
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