Spectrometer scans, data collection, and processing were controlled by a personal computer.
Each B-scan data collection distance corresponds to approximately 1 m width.
Hybridization, washing, staining, scanning and data collection were performed at the Genomics Core Facility, Pennsylvania State University, University Park.
Hybridization, washing, staining, scanning and data collection were performed in Genomics Core Facility at Pennsylvania State University.
For FLIM investigations described herein, scanning and data collection were performed using commercial time-correlated single photon counting (TCSPC) hardware (SPC-150, GVD-120, DCC-100, Becker & Hickl GmbH) and control software (SPCM, Becker & Hickl GmbH).
Two practicing clinicians (alternatively described as examiners or observers A and B in the manuscript), trained in the use of paraspinal thermal scanning carried out all scans and data collection on all study subjects.
Students gain first-hand exposure to the scanning environment, data collection procedures, and basic, hands-on experience with data treatment and statistical analysis.
Chip scanning and data collection were performed with the Affymetrix GeneChip Operating Software (GCOS) version 1.4.
However, the mechanical requirements of sequential scanning slow data collection and pose a challenge for real-time imaging.
The resultant cartridge was then transferred to the nanoString nCounter digital analyzer for scanning and data collection.
All protocols for microarray sample preparation (except total RNA extraction, as above), Affymetrix Rat Genome 230 2.0 GeneChip® hybridization, array scanning and data collection were performed by Expression Analysis, Inc., (Durham, NC) according to standard Affymetrix protocols.
