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The participant was positioned in the prone position and one breast at a time was placed within the scanning chamber which was filled with water.
A third motor (Flex Drive Baldor, Fort Smith, AR, USA) rotates the whole scanning chamber continuously from 0 to 180° at each scanning plane and a fourth motor (R88m Omron/Yaskawa, Kioto, Japan) elevates the scanning chamber in steps of 4 mm between scanning planes.
An automated control system assures the safety of this initialisation, followed by lowering the scanning chamber by 16 mm before starting the scan.
The breast is positioned through an opening into the scanning chamber (1).
To standardise this position, the scanning chamber is raised until it touches the Lexan.
The other cycle transports the water from the tank into the scanning chamber using another pump (SE-PSH000HM1000, SEKO Spa, Italy) with a maximum flow rate of 2 l/min and once more passes an identical UV-C lamp.
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Occasionally, Poxn-pRes males were scanning the chamber when losing track of the dewinged object male (M23) in a fashion similar to that observed for heterosexual courtship in the dark (second part of Movie S3).
Between injections, a CCD camera captured high-resolution images, and an ERT data acquisition system scanned the chamber.
We then scan the chamber laterally through a 3D motion controller (ESP300, Newport) to search for individual EGFP molecules and simultaneously use an EMCCD camera (Evolve, Photometrics) to collect the emitted fluorescence photons with an exposure time of 1 s.
The key parts were purchased from Oxford Microbeams Ltd., including triplet quadrupole lens (model OM-150), collimator slits, scanning system, target chamber, and data acquisition system.
He stood naked on top of the vice president's desk in the Senate chamber, scanning the crowd of sniveling politicians below him.
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