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Subsequently, US scanning by using a low-MI (≤ 0.1) technique, CnTI (Contrast tuned Imaging; Esaote, Genoa, Italy), was performed to ensure sufficient enhancement after bolus administration, allowing an examination window of up to 5 minutes.
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We have developed a comprehensive image-driven segmentation methodology to segment the cardiac structures (or only the left heart) from CT scans by using a processing pipeline of multi-thresholding, image cleaning, mathematical morphology, and image filtering techniques.
The arrays were washed and scanned by using a ScanArray 4000 (Perkin Elmer).
Respiratory movements were recorded continuously 2 min before, and during each scan, by using a strain gauge around the lower thorax connected to a graph (Comair, Stockholm, Sweden) as described previously [9].
Micro-arrays were scanned by using a confocal laser scan system (Scan Array 4000, General Scanning).
The reference arm was scanned by using a corner cube prism mounted on a galvanometer.
Processed chips were scanned by using a Perkin Elmer ScanArray® XL 5000 Scanner, software version 3.1.
Arrays were scanned by using a 4000B Scanner (Axon Instruments, Union City, CA, USA).
Gels were scanned by using a digital photograph system (Gel Logic 100, Eastman Kodak, Rochester, NY, USA).
The hybridized slides were scanned by using a GenePix Personal 4100A Microarray Scanner (Axon Instruments).
The total spots and immunoreactive spots were scanned by using a Molecular Imager FX laser scanning fluorometer (BioRad Laboratories, Hercules, CA).
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