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Scanning and quantitation was as given above.
The authors thank Dr Bart Frank (OMRF) for help with protein array scanning and quantitation.
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The mass spectrometer operated for quantitative analysis in full scan MS/MS and quantitation was based on post processing MRM extracted ion chromatograms.
Microarray experiments are composed of sample preparation, hybridization, scanning and image quantitation, which are a series of integrative procedures being conducted at a laboratory, generally according to the manufacturer's recommended protocols.
Importantly, PET tracer development and imaging of protein aggregates require different approaches to those involved in imaging of receptors or transporters, including lead optimisation, scan analyses and quantitation.
Immunoblots were scanned and protein quantitation determined with Metamorph v7.7 (Molecular Devices, Sunnyvale, CA, USA) by computing the integrated density values (idv) of non-saturating bands corrected against the background.
Hybridization is detected by incubation with streptavidin-phycoerythrin conjugates, followed by scanning the array for phycoerythrin fluorescence and quantitation.
Scans were performed on Typhoon 8600 Variable Mode Imager (Molecular Dynamics), and quantitation done using ImageQuant.
Two dimensional gels of whole cell protein are scanned using a Bio-Rad QS-800 Calibrated Densitometer Gel map imaging and quantitation.
A combination of X-ray diffraction (XRD), the Rietveld methodology, differential scanning calorimetry (DSC -thermogravimetric (TG) analysis anDSC -thermogravimetriccroscopy (SEM)-energy dispersive specTGanalysisis (EDS) wanduscanningnvelectron the comicroscopyand quantitation of clinker phaSEM -energy
The detection and quantitation of target hybridization was performed with a GeneArray Scanner (Hewlett Packard/Affymetrix) set to scan each array twice at a factory set PMT level and resolution.
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