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After cDNA synthesis (1C1), the T-array protocol follows PCR amplification (1C2), isolation of polyclonal, single strands (1C3), hybridization of annealer oligonucleotides (1C4), and ligation, washing, scanning, and quantification of hexamer arrays (1C5 C7).
Slides are blocked, blotted, and incubated with antibodies before measuring signal intensity by scanning and quantification of results.
Scanning and quantification of the results were done using a Fuji FLA 9000 imager, followed by analysis using MultiGauge software (Fuji).
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All blots were scanned and quantification of the relative intensity of each band was evaluated by using the NIH Image J program (http://rsb.info.nih.gov/ij/index.html).html
Films were scanned and quantification of the bands was performed using Image J software http://rsbweb.nih.gov/ij/.
Differential fluorescent labeling of internalized vs. bound bacteria or beads allowed automated image analysis of collapsed confocal stack images acquired by scanning cytometry, and quantification of total particles bound and percent of particles internalized.
Scanning, analysing and quantification of blots were performed via the Odyssey Infrared Imaging System (LI-COR Biosciences).
Immediately prior to urgent endotracheal intubation in ICU patients, ultrasound scanning enabled useful identification and quantification of stomach fluid in patients.
This underlines the need for multicenter standardization of scanning and quantification.
Fluorescence was detected with the laser scanning confocal microscope (Zeiss, Germany) and quantification of the immunostaining images was performed by the blind cell counts 10-200 sectiors or > 1 000 cells per group) using the ImageJ software.
The current retrospective data analysis was undertaken to examine F-FDG-avid lesions and corresponding background tissues on same-day preoperative and postoperative F-FDG PET/CT scans to assess the feasibility and quantification of diagnostic F-FDG PET/CT oncologic imaging at extended injection-to-scan acquisition time intervals.
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