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Product-ion scanning analysis was repeated n-times for molecular ions of m/z = (100+n), n = 0,1,2.251 at a scan width of 0.5 each.
Densitometric scanning analysis was performed by Mac OS X (Apple Computer International), using NIH Image 1.62 software.
Immuofluorescence double staining and semi-quantitative confocal laser scanning analysis was carried out to detect nuclear factor κappa B nuclear localization and staining intensity of VEGF.
Densitometric scanning analysis was performed on Mac OS 9.0 version, using NIH Image 1.62 software, developed at the U.S. National Institutes of Health [ 22].
Therefore, a scanning analysis was performed with a series of annealed complementary oligonucleotide probes in which the coresequences were changed to CCGG.
Confocal scanning analysis was performed by using a Leika laser confocal scanning microscope (Solms, Germany) in accordance with established methods, using sequential laser excitation to minimize the possibility of fluorescent emission bleed-through.
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Moreover, motif scanning analysis is limited to motifs that are available in databases, which are incomplete.
All processing steps like DNA labeling (Cy3 for samples and Cy5 for references), array hybridization, data normalization and scanning analysis were performed following standard procedure.
Full scan analysis was recorded in m/z range from 100 to 2000.
In group II (3 knees), CT scan analysis was also done on Thiel embalmed cadavers.
The spatial scan analysis was constructed using postal code data for participants (n = 154) residing in the Vancouver Metropolitan Area.
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