Sentence examples for scaled up the expression from inspiring English sources
In order to determine if proteins expressed from the synthetic genes could be used successfully in protein crystallization experiments, we scaled up the expression and purified full length and truncated forms of FtsZ.
There are two common strategies to scale up the expression of protein: one uses cells grown in suspension in the Fermentor/Bioreactor and the other uses large tissue flasks with adherent culture.
In order to scale up the co-expression system to allow detailed analysis of the complex, the original torAD SPA allele was cloned into expression vector pQE80 and the DNA coding for the SPA tag at the C-terminus of TorD replaced with a hexahistidine tag.
These reflect a baseline equipment size which was scaled up or down according to the exponential scaling expression (Equation 1) [ 40], where the f scale represents a scaling exponent specific for each piece of equipment, usually ranging between 0.6 and 0.7: 1 All costs were indexed using the Chemical Engineering Plant Cost Index to the reference year of 2011 chosen for this study [ 40].
An obvious next step would be to scale up the production of antibodies through recombinant expression of the identified immunoglobulin genes in suitable cell lines.
Optimal conditions for rGRA5 protein expression in E. coli were determined prior to scaling up the protein production protocol for further study.
Ever since, the field investigation scaled up.
Recently Davies et al., 2005 [ 24] described a method for transient transfection of HEK-293E cells in 24-well blocks with a lipid based transfection reagent and validated this by comparing with scaled up shake-flask expression for a set of intracellularly expressed kinases.
Why is scaling up the model desirable or necessary?
We have recently shown that TaqMan® RT-PCR can be scaled up to profile expression for 192 genes in fixed paraffin-embedded (FPE) clinical study tumor specimens.
