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In this study, we carried out a large scale of expressed sequence tags (ESTs) sequencing from developing sesame seeds and further conducted analysis on seed storage products-related genes.

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Until recently, oomycetes genomics focussed only on Phytophthora spp. with the completion of genome sequencing projects and large scale identification of expressed sequences [12] [17].

To overcome this hurdle, the current gene discovery project was initiated with an objective of isolating as many functional genes as possible from J. curcas by large scale sequencing of expressed sequence tags (ESTs).

Although the RPE cell is one of the best studied neural cell types, [ 3- 12] large scale assignment of expressed genes to the RPE has been largely dependent on RNA based studies.

Large-scale analysis of expressed sequence tags (ESTs) should help us to clarify the overall expression of genes in the male strobili of C. japonica.

The DNA fragments were amplified from cDNA clones generated as part of large-scale sequencing of expressed sequence tags (ESTs) in the Sino-Danish Pig Genome Sequencing Project [27].

The increasing efficiency of DNA sequencing has enabled us to assemble large-scale collections of expressed sequences (ESTs: Expressed Sequence Tags) from spider silk gland cDNA libraries.

An alternative strategy for gene discovery involves the large-scale sequencing of expressed sequence tags (ESTs) randomly selected from cDNA libraries prepared from many different human tissues and cell lines.

Since large-scale analysis of expressed sequence tags (ESTs) in the male strobili of C. japonica should help us to clarify the overall expression of genes during the process of pollen development, we constructed a full-length enriched cDNA library that was derived from male strobili at various developmental stages.

The large genomes of conifers have greatly hindered their sequencing (but see [ 5]), but they have also prompted large-scale investigations of expressed gene sequences for the inference of putative unigene sets (reviewed by McKay and Dean [ 6]) and initiatives to map these genes (reviewed by Ritland et al. [ 7]).

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