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However, the standardization of the absolute scale of assays remains problematic, and it is evident that direct assays consistently overestimate estradiol or other sex steroid concentrations.
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ADMIREs statistical approach permits simultaneous large-scale analyses of hundreds of assays with little impact on algorithm runtimes.
Although sensitive for the detection of certain phenotypes, such as alterations in movement or morphology, manual inspection of plates or images is time consuming and tedious for HTS campaigns scaled for assaying hundreds-of-thousands of compounds [28], [29], [33].
Targeted peptide-based protein assays allow large-scale, quantitative proteomic analysis projects and have found widespread application in the systematic development of assays for protein biomarker validation studies.
ATLAS can be used at the scale of individual assays and high-throughput approaches.
Therefore, there is 20-fold difference in the available viral RNA or the infectious units between the RT-LAMP system and the cell culture system because of the intrinsic difference of the scale of the assay (12.5 μl vs. 1.0 ml).
To our knowledge, only two other laboratories have reported the use of miniaturized scaled ChIP assays in loci-specific and global microarray-based surveys [ 26, 27].
We chose to compare the effect sizes because of different scales and assays used in each study and because the standardized effect size conveys the size of an effect relative to the variability in the different populations (Hartung et al. 2008).
Large-scale mining of assay data has revealed different facets of promiscuity.
Our experiments establish that GV and other tzs containing strains are a constant source of significant amounts of cytokinin when infiltrated into leaf tissue, and that this is the cause for the observed changes to plastid morphology in the relatively short time-scale of transient assays.
JBo participated in scaling PCR assays, data analysis, manuscript writing, and co-funding of the study.
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