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Other developments needed for widespread hPSC-CM utility include subtype specification, cost reduction of large scale differentiation and elimination of the phenotyping bottleneck.
At a finer scale, differentiation in DTDFs was higher between diets (roach fish vs chick) than among tissues: ΔN was more enriched in the chick diet than in the roach fish diet, and the inverse was true for ΔC.
Despite this strong differentiation among colonies and years at a local scale, differentiation of S. bechsteini between colonies from spatially distinct regions was not higher than within regions, suggesting that overall genetic diversity of the S. bechsteini population at a regional level is Stable.
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I refrained from defining characters that, although widespread, involve much subjectivity when coding, or for which the homologisation cannot be identified (reliability) such as the shape and development of pigmentation, detailed carapace or scale differentiations – all characters important for staging pre-hatching/pre-birth embryos of a particular species.
Examples of excluded characters are the shape and development of pigmentation, detailed carapace or scale differentiations – all characters important for staging prehatching embryos of a particular species.
Biomedical application of human pluripotent stem cell-derived hepatocyte-like cells (hPSC-HLCs) relies on efficient large-scale differentiation, which is commonly performed by a suspension culture of three-dimensional (3D) multicellular spheroids in bioreactors.
The 491-bp stretch amoA gene has strong capacity for fine-scale differentiation of closely related ammonia oxidizers and has been utilized as a functional gene marker for identification of ammonia-oxidizing microorganisms (Rotthauwe et al. 1997; Wang et al. 2012).
Complete genealogical exclusivity is, of course, unexpected for Holocene-scale differentiation in animals.
The fine-scale differentiation between individual EHDV strains within each serotype and topotype depends on the availability and characterisation of well documented isolates, such as those stored and maintained in the reference collection at IAH Pirbright [48], [49].
Regionally restricted gene flow in the Caribbean Acropora argues for regionally-based management [20], [21], but evidence for additional fine-scale differentiation in A. cervicornis suggests that the scale of dispersal and thus management at some locations may need to be much smaller (i.e. on the order of individual reefs) in this species [21].
The large-scale differentiation into neurons, axon regeneration, and extensive synaptic contacts reformation with host motor neurons was observed.
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