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The threshold value was set within the exponential phase of the logarithmic scale amplification plot.
Large scale amplification and deletions have been known for several decades [ 39- 42].
DNA microarrays are mainly used in model plants as they generally require either extensive gene sequence information or large scale amplification of cDNA libraries.
The most critical step in the SAGE method is large scale amplification of ditags which are then are concatemerized for the construction of representative SAGE libraries.
For reasons of practical handling, all further experiments were carried out using only Sf21 cell lines, for small scale amplification, and Sf9 cell lines, for large scale amplification (Sf9 cells being more suitable to this because they were maintained as a shaking culture versus Sf21 cells that were maintained as a monolayer culture).
Before USSCs can be used therapeutically, a method for large scale amplification of cells under Good Manufacturing Practice GMPP) conditions is necessary, preferably using defined medium free of xenobiotic components.
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Despite the large-scale amplification, Ms. Futral manages to project sensitivity, vulnerability and, during the famous Act III mad scene, an eerie detachment from reality.
But orchestral strings do not respond as well as the human voice does to large-scale amplification, and however appropriate it was to be hearing the first movement, "Prelude to Night," as the stars were starting to peep out, the fine textures collapsed almost into homogeneity.
Here we describe the KeyPoint™ technology, a novel mutation/polymorphism screening technique using the Genome Sequencer (GS) FLX platform (Roche Applied Science), which allows massive parallel picoliter-scale amplification and pyrosequencing of individual DNA molecules [20].
For the large-scale amplification step, a total of seven 25 µl PCRs were performed using exactly the same conditions but the optimal cycle number, as determined in the initial titration experiment.
Both methods agreed on significant large-scale amplification events for the whole chromosomes 7, 19 and 20 and focal amplification events on chromosome 1 and 12; significant large-scale deletion events on chromosomal arms 6q, 9p, 15q, on whole chromosomes 10, 13, 14 and 22; and focal deletion events on chromosome 1.
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