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However, the lack of scalability of production due to the relatively high volatility and strong toxicity of the dispersion solvent have created a great need for environmental friendly process for expanding TE applications.
One current advantage of γRV systems is the availability of stable vector packaging cell lines (Miller & Buttimore, 1986), allowing for ready scalability of production, whereas LV stocks are produced transiently, by transfection of multiple plasmids into permissive cell lines.
Hereby, we describe for the first time the successful heterologous expression of a PDH gene in the methylotrophic yeast Pichia pastoris and the scalability of production and purification of the enzyme on larger scale.
Numerous studies have demonstrated the cost-efficiency and the scalability of production of many recombinant proteins, which include diagnostic reagents [ 13- 17], vaccines [ 14, 18- 26] and therapeutic molecules [ 27], among others.
In addition, the scaling up process is, at times, complicated by decisions made early in the development process, when research is typically underway in an academic setting and relatively less attention is paid to the scalability of production and related costs.
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In the present work, the scalability of the production process will be discussed.
Taken together, plant-derived VLPs are considered safe because plants do not bear human pathogens and promising in terms of cost-effective scalability and speed of production.
The only system that can rival LTE in terms of scalability, yield and production cost is the E. coli cell-free system [15].
Guidelines for realizing specific sheet attributes, and scalability of LSEM for production are analyzed and discussed.
The modest running costs in conjunction with the scalability of agriculture-based production systems highlight the potential of transgenic plants as "green factories" (Dyer et al., 2008).
Check the scalability of the product over next few years.
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