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The dehydrated scaffold sections were placed in vacuum desiccators for complete drying.
After mild washing with PBS, the scaffold sections were dehydrated by gradually increasing concentration of ethanol (20%, 40%, 60 %, 80, and absolute ethanol) for 10–15 min each.
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In the scaffold section, engineered products as well as solutions that use natural tissue will be discussed.
In the ECR browser, a scaffold section known to contain the NCX4 gene flanked by a minimum of 30,000 base pairs was used to align with all other available genomes to find synteny patterns.
H&E staining of decellularized-scaffold section shows collagenous ECM structure with negative cell nuclei staining), which indicates the nonexistence of any cell in the scaffold.
Bone formation was quantified through histological analysis, and reported as a percentage, area bone/area cross section scaffold × 100.
The remainder of the scaffold comprises sections of gene-free single copy DNA and repetitive elements.
White sections in Figure 3A represent those sections of SN15 scaffold 2 that are annotated as coding sequence, whereas B and C represent the mapped read density of Sn4 and Sn79, respectively.
A Leica VT1000 S Vibratome was used to section these blocks at a thickness of 200 μm, to reveal the scaffold cross-section.
To show the distribution of cells within the scaffold, z-sections were made at different positions after 14 days of culture.
The microbeads and scaffold cross-sections were observed at an acceleration potential of 5 kV and 10 kV, respectively.
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