Based on the dysmorphic phenotype of the Hnf6 Tg islets, we expected to see alterations in factors involved in cell adhesion, cytoskeletal/ECM molecules, and protease/protease inhibitors.
In our study with sporadic PTC, we did not see alterations in SDHB at the mRNA level, but rather significant reductions in SDHC/D gene expression.
Most of the genes affected represent those involved in transport but we also see alterations in genes encoding proteins of unknown functions.
Given that we had expected to see alterations in MBNL target transcript splicing, we sought out additional potentially dysregulated splicing events in injected zebrafish.
This is in contrast to the work of Schintu et al. (2009) who did not see alterations in MPTP bioactivation following rosiglitazone treatment.
Although flies lacking Arl5 are viable and fertile we could see alterations in the endosomal system consistent with sub-optimal traffic from endosomes to Golgi.
While we do not see alterations in the levels of a housekeeping factor, 16S rRNA, in wt and Δ rpmC cells, we cannot rule out the possibility that altered mRNA levels for other cellular proteins may be partly responsible for the changes in target protein expression seen here and is a topic that warrants further investigation.
Furthermore, the authors' failure to see alterations in levels of CNP-C, -T or Ndc80 when the minimum "critical" threshold level of CENP-A is passed suggests that the explanations for changes in the micronucleus index that they see when CENP-A levels drop below 50%.
The present study was designed to see alteration in pulmonary function tests i.e. spirometric parameters and blood oxygen saturation consequent to adenotonsillar hypertrophy and subsequent changes after adenotonsillectomy.
For both, l y75- and adora2a-morphants, we saw no alterations in molecular chamber specification and expression of atrial and ventricular myosin heavy chain genes (Figs 8 and 9 and Supporting Information Fig 4).
