Sentence examples for save pellet from inspiring English sources

The homogenate to which 10 mM MgCl2 was added was allowed to incubate on ice for 15 min followed by two centrifugations, the first at 3000  g for 15 min at 4°C (discard pellet 3) and the second at 27,000  g for 30 min at 4°C (save pellet 4).

The initially saved pellet was then resuspended into the harvested concentrate.

The supernatant was saved, pelleted at 10,000 g for 5 min, and designated as non-cardiomyocyte fraction.

To collect and save the pellet from the PBS extraction, the pellet from one tube was resuspended with 100 μl PBS and saved.

The supernatant was saved, the pellet resuspended in the same volume of mito buffer supplemented with 0.1 mg/mL digitonin, homogenised and centrifuged once again.

The supernatant was saved; the pellet was resuspended in 1/3 of the initial volume, and centrifuged again at 1,000 g for 10 min.

After centrifugation (4500 g for 3 min at 4°C), supernatants were saved and pellets were washed with lysis buffer three times.

The supernatant was saved and the pellet was discarded.

The supernatant 1 (S1) was saved and the pellet resuspended in the lysis buffer.

The supernatant was saved and the pellet rehomogenized in 2.5 ml buffer A and centrifuged as above (280×g, 5 min).

The supernatant was saved, and the pellet was resuspended in 100 μl of sucrose binding buffer.