The gain and offset of the PMTs were adjusted for optimized detection using the color gradient to avoid pixel intensity saturation and background and these settings kept constant for all measurements.
You can always bump saturation in the settings, though.
Hemodynamic, respiratory, and blood sample parameters: arterial blood pressure, heart rate, vasoactive drug doses, pulse-oximeter oxygen saturation (SpO2), ventilator settings if intubated, arterial and central venous blood gases and acid base status, lactate, hemoglobin, and axillary temperature.
It is estimated that approximately twenty semi-structured interviews with NHS professionals and up to four focus groups with service-users is likely to achieve data saturation in the settings identified.
Cells with very high levels of GFP expression (saturation at gain settings >650) often displayed aberrant morphology and/or might be expected to have large pools of free GFP-tagged protein due to saturation of potential binding sites and were, therefore, excluded from selection for bleaching.
The difference in expression levels of JAM-C in these two structures is clearly demonstrated in Fig. 4 D, where, at saturation image-acquisition settings in WT tissues, the high JAM-C fluorescence signal detected in E-cadherin+ SCs surrounding individual axons (indicated by arrows) makes the detection of the low- JAM-C-expressing perineural cells surrounding nerve bundles (arrowheads) difficult.
All microscope settings were set to collect images below saturation and were kept constant for all images taken in one experiment, as previously described [ 26].
Images were acquired using the same settings below saturation at a resolution of 1024X1024 pixels (12 bit).
Stroke volume variations and oxygen saturation values guided ventilator settings until extracorporal membrane oxygenation could be arranged for.
In the meantime the arterial blood pressure and heart rate were stable and echocardiography together with oxygen saturation guided the ventilator settings.
