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However, it should be noted that addition of glucose causes saturated cultures to become very acidic, limiting saturation density (Studier 2005).
The parameterfree strength of the 2-term, F∇ kf), is at saturation density comparable to that of phenomenological Skyrme forces.
The quantitative analysis of the current versus time transient responses, on the time scale of seconds, indicated a low saturation density of nuclei of 2×106 cm−2.
A linear relationship between the nucleation rate and the inverse of induction time was found in each experimental condition and their slopes are related to the saturation density of the nuclei.
We find that in the region below the nuclear matter saturation density
1990a), where S, ρ, η andκ denote saturation, density, viscosity and permeability, respectively, g is the gravity constant, i indicates the spatial variables x(1), y(2) and z(3),δ ij is Kronecker's delta and the subindex "i" denotes spatial differentiation.
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In contrast, CR rat serum-treated cells during the same time period retained fibroblastic morphology and attained higher saturation densities, indicating that CR serum was able to delay the passage-induced senescent phenotype (Fig. 1B, lower right panel).
However, no correlation was found between the growth rates in vivo and either the doubling times and saturation densities in monolayer cultures or the plating efficiencies in soft agar.
Several studies showed that overexpression of different PKC isoforms induces high growth rates, cellular saturation densities and enhanced tumorigenicity (Housey et al, 1988; Persons et al, 1988; Cacace et al, 1993; Mischak et al, 1993; Borner et al, 1995).
Saturation densities were typically lower than what we could observed for TOV112D, being between 25%to50%0% of the value obtained for TOV112D (Table 2), and more in line with the previously densities described for other serous ovarian cancer cell lines, such as TOV1946 and TOV2223 [ 19].
Similar manifestations of density dependent inhibition were found in the isolated cultures of normal and neoplastic cells: at saturation densities these cultures had low labelling indices; these indices considerably increased when the cells migrated into the wound from the dense sheet, prelabelled cells seeded on the dense sheets of unlabelled homologous cells did not proliferate.
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