Previously published work concluded that at high water saturation, water blocking significantly affects the trapped volume of oil, but that oil trapping is insignificant after large pore volumes of injections (Salter and Mohanty 1982; Tiffin and Yeilig 1983; Wang 1988; Lin and Huang 1990; Zekri et al. 2006).
The difference lies in the simulations of saturation through blocking.
In practice, the bags did not dissolve completely, leaving fragments of plastic in the saturation tank, blocking the circulation pumps, accumulating on the base of the tank and inhibiting the injection of fluoride into the water supply.
This indicates that gel was formed in the high-permeability sand pack due to the high CO2 saturation and blocked the channels and pores.
The day before the recording, OP50 were grown to saturation then blocked for 40 minutes at 37°C with streptomycin at 50 µg/ml.
The saturation magnetizations and blocking temperatures of the samples were measured using a Quantum Design MPMS-7 superconducting quantum interference device (SQUID) magnetometry.
We used SQUID magnetometry to measure the saturation magnetization and blocking temperatures of each preparation of SIPPs. Figure 7 shows the hysteresis curves for each SIPP sample, as well as the ZFC/field-cooled (FC) curves.
Sheep anti-rabbit IgG Dynal beads (Invitrogen) were prepared by washing 2× PBS and 2× IP buffer, before bead saturation overnight in blocking solution (IP buffer, EDTA-free protease inhibitor tablet, 1 mg/ml BSA).
After saturation with BSA blocking solution (Candor, Germany), the plates were incubated with either samples, recombinant soluble MICA*04 or recombinant ULBP3-Fc (1517-UL-050, R&D Systems, USA) as standards.
Saturation is simulated through blocking the available paths.
Following two washes with PBS, saturation was achieved with blocking buffer (PBS, 10% donkey or goat serum depending on the secondary antibody, 50 μg/mL human IgG) for 30 min at room temperature.
