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Competition analysis was then performed on all mER subtypes by incubating lysate with a saturating concentration of [3H]E2β (2–3 nM, determined from saturation analysis) and a range of competing analyte concentrations.
The methodology estimates the fission product inventory in the core based on saturation analysis method.
We examine in some detail the procedures and justifications of Wheeler's sensitivity analysis and relative rate comparison (saturation analysis).
When saturation analysis of [3H]-nicotine to THP-1 cells was performed in the presence of 1 × 10−6 M epibatidine, only one binding site was detected.
Both libraries reached approximately 63 65% sequencing saturation (see saturation analysis in supplementary material, Figure S2).
The same 4007 expressed genes and 2134 unexpressed genes in MCF7 cells were used for saturation analysis.
To assess whether the RPol II sequencing depth is adequate in identifying active promoter regions, we performed saturation analysis by analyzing how the prediction power change when only a subset of sequencing reads are used for prediction.
Saturation analysis indicates a Bmax of 151.11±21.47 nCi/mg protein and Kd of 194.64±36.92 nM in the MGB (mean ± SEM). Figure 1 shows representative coronal sections through the MGB, displaying lower levels of [3H]gaboxadol binding in hippocampus.
Sequencing saturation analysis.
The sequencing saturation analysis.
C: saturation analysis for sequencing library.
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