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BLAST results were parsed using PERL scripts in order to select only those orthologs satisfying alignment criteria defined in literature [ 14]: alignment extension over 70% and e-value cut-off 10-5.
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The highest scoring alignment satisfying the stringency criteria was taken as the best hit.
BBH are defined as gene couples encoding proteins that are considered orthologous based on either reciprocal best BLAST hits or satisfying a BLASTp alignment threshold (a minimum of 35% sequence identity over 80% of the length of the smaller protein).
The 'most suitable' primer tuple is defined as the tuple with the most similar score to the average score of the current primer tuples set, while satisfying cross-tuple alignment constraints as defined by the user.
The calculated electronic properties of the codoped nanotubes evidence the widely tunable band gap, enhanced mobility, and better band edges' alignments, satisfying basic prerequisites as a new class of high efficiency photocatalysts for water splitting and other avenues of solar energy utilization.
We retained only the alignments satisfying the thresholds of 95% identity and 90% coverage.
A given sRNA was determined to be present in an E. coli or Shigella target genome if BLAST searches yielded one or more alignments satisfying both E value < 0.001 and target length ≥ 0.7 × query length.
A sRNA-encoding gene was considered to be present or conserved in a given genome if BLAST searches yielded one or more alignments satisfying the similarity criteria outlined in Methods.
To obtain the alignments showing unambiguous orthology, we extracted the alignment blocks satisfying dHC < 0.05, dHCO < 0.08, and the null hypothesis of dH = dC for a relative rate test (Tajima 1993), where dHCO = (dHO + dCO)/2 and dH and dC represent the evolutionary distances from the branching point between human and chimpanzee to their leaves, respectively.
SINA uses a combination of k-mer searching and partial order alignment (POA) to maintain very high alignment accuracy while satisfying high throughput performance demands.
Alignments not satisfying either of these criteria were excluded.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com