With one exception, all of the primer pairs amplified products in which there was a clear, predominant band, and thus served as satisfactory templates for sequencing.
All host DNA samples retained for analysis were positive for PCR amplification using the 18S rDNA arthropod universal primers, indicating satisfactory DNA template quality (less than 1% of DNA templates failed to amplify the 18S rDNA fragment by PCR).
Although the efficiency of binding-sites prediction for unbound chains has been significantly increased benefiting from Homology Indexing, there are still some chains of no satisfactory homologous template structures, such as PDB ID: 4h12, CHAIN ID: A. For this kind of protein chains, we further introduce Chain Length Indexing to reduce the number of time-consuming structural alignments.
Positive amplifications of other loci (pk1, ank2) from samples with null alleles at either MutL, GP15 or RepA loci indicate satisfactory DNA template quality in these cases.
Meanwhile, the obtained polymers have high capacity of 12.87 mg g−1 and satisfactory selectivity to template molecule.
Meanwhile, the obtained polymers have good capacity of 6.67 mg g−1 and satisfactory selectivity to template molecule with the imprinting factor of 5.11.
If the query chain has no satisfactory homologous bound template, the binding site of this query chain will be detected as the following procedure.
The implant was only indicated for patients at a maximum age of 65 years and a physiological preoperative CCD-angle, where the implant could be placed satisfactory in preoperative templating.
Since there are still lots of protein chains have no satisfactory bound structures, bound templates is borrowed for detecting the binding sites in this situation.
and found that phenol based DNA extraction was by far the most satisfactory, consistently producing good quality template for our qRT-kDNA PCR diagnostic assays.
Samples containing an optimum number of template ISPs and satisfactory enrichment were subjected to the standard Ion PGM™ 200 Sequencing v2 protocol.
