Sentence examples for satisfactory staining from inspiring English sources

Once satisfactory staining was obtained the staining solution was removed completely and samples were washed three times in PBS-T for 5 min per wash while remaining in the wells.

FGF2, SMOC1 and TIMP2 were chosen for IHC testing, as these genes had markedly increased RNA expression levels in BPH relative to controls, and antibodies were available that produced specific and satisfactory staining of human TZ tissues.

We also obtained satisfactory staining for fms-like tyrosine kinase 3/fetal liver kinase 2 (Flt3/Flk2) and CD48, either of which can further be used to discriminate between HPCs, MPPs and LT/ST-HSC [12] [14] (Figure S2, Materials and Methods S1).

Of the 94 specimens, satisfactory staining was achieved in 86 cases (91.5%).

One of the positive tumours was selected as a biological positive control and included in each run, ensuring satisfactory staining reproducibility.

The sections were then placed in a light-protected environment with approximately 400 μL of BM-purple AP substrate (Roche) until satisfactory staining was achieved.

Finally, we found that cryopreserved MHC multimers can be stably stored at both −20°C and −80°C over a period of at least 6 months, leading to satisfactory staining-efficacy for a number of complexes for up to one year (Fig. 4).

Satisfactory immunohistochemical staining was obtained in 90 cases, and these cases were enrolled in this study.

It was not possible to obtain satisfactory dual staining for intracellular IL-17 and the macrophage marker CD68 on archival material.

After staining optimization by using positive and negative controls, eight antibodies that provided satisfactory results, together with standard prognostic biomarkers, including ER, PR, HER2, and Ki-67, were used for the study (Additional file 1).

Some studies found that the efficacy of H&E staining alone in diagnosing PV is probably greater than 90% and could be considered satisfactory [ 92, 93].