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Between three and nine assemblies are run until a satisfactory assembly is produced.
Overall, our current work suggests that satisfactory assembly quality can be achieved using the Nextera XT kit down to 5 picograms of input DNA with little effect on downstream analyses such as extracting genomes from metagenomes (Fig. 5).
If one of these runs does not produce a satisfactory assembly, then two minimum molecule length variants (180 and 100 kb) are tested with the p- V a l u e T h r e s h o l d of the current best assembly (Fig. 2 g).
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Another two clones (565E21, 575D20) have considerably longer inserts than the others (>180 kb compared to ~110 kb as proved by restriction fingerprinting analysis) and were therefore not sufficiently covered by 454 reads to get satisfactory assemblies.
The closer the fit on the original cut, the less hand fitting will be required to make the final assembly satisfactory.
When using IIPS, the user has a set of tools and needs to know how to use each one to get a satisfactory result in the assembly site.
The reliability of press-packed insulated gate bipolar transistors (IGBTs) depends on satisfactory contact conditions applied at assembly stage and maintained throughout the service life.
These results suggested that the final assembly was highly satisfactory.
PCR based amplicon end sequencing resolved all the gaps bringing the sequence assembly to a satisfactory level of completion.
This genome was recently sequenced using a fosmid-pooling strategy after whole genome assembly failed to produce satisfactory results (Zhang et al., 2012).
This combined sequencing and assembly approach resulted in satisfactory analysis parameters since it improved overall coverage and read length, slightly increased the genome size (2.928 - 3.33 Mbp) and reduced the number of genomic contigs (<100; Table 5).
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CEO of Professional Science Editing for Scientists @ prosciediting.com