Sand-maize meal inoculum of different isolates was then mixed together in sterilised 1000 ml conical flasks.
The sand-maize meal cultures were then incubated at room temperature for 6 weeks, with shaking once a week for even colonisation.
Sand-maize meal inoculum of the take-all fungus was prepared by first filling 500 ml conical flasks with 100 g horticultural grade silver sand, 3 g maize meal and 10 ml of distilled water.
A control treatment without addition of Ggt sand-maize meal was set up with the winter wheat cultivar Hereward to ensure the soil used was free from take-all.
A mixture of 150 g take-all free soil, 100 g damp coarse sand and 50 g of a 1 50 dilution of sand-maize meal Ggt inoculum in silver sand (mixed in a plastic bag) was used in the pot test.
Further, the fungal mass culture was prepared by adding the spore suspension to the autoclaved mixture of sand and maize bran (19 : 1) and incubating it at room temperature for 4 weeks.
The long-term impact of tillage and residue management on soil microorganisms was studied over the growing season in a sandy loam to loamy sand soil of southwestern Quebec, growing maize (Zea mays L). monoculture.
Moreover, Montezano et al. 2006 observed that soil property such as sand content was negatively correlated to maize yield.
OPUWO, Kunene region, Namibia, 7 August 2013 – Kariamakuju Kauta lifts some dry, dusty sand from what was a field of maize and vegetables.
Positive linear correlations observed between maize yield and pH, EC, K, and sand indicate that these properties were strongly correlated with maize yield.
Maize seeds were germinated in either autoclaved sand or on filter paper, moistened with deionized water, for 3 5 d at 28°C in continuous darkness.
