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Before and during the PET study, nine arterialized venous blood samplings were obtained: at background, 30 sec, and 1, 3, 5, 10, 20, 30 and 50 min after the tracer injection from the antecubital vein contralateral to the intravenous FBPA injection side, heated in a heating blanket.
Samplings were obtained at the meteorologic station of the Escola Superior de Agricultura Luiz de Queiroz, which is located about 4 km from downtown Piracicaba and < 1 km from the nearest sugar cane plantation.
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On all sampling days after day 0, samples were obtained only from tester animals.
PCR-positive samples were obtained from all sampling locations, with the highest detection rate in the valley.
A total of 1999 bootstrap samples were obtained by re-sampling men from the study sample with replacement, so accommodating the repeated measures design and any non-normality in the distributions of outcome measure.
To minimise sampling error at least six biopsy samples were obtained from different sites in the joint during each procedure.
A second sample was obtained with a sampling device and suspended in 20 ml of the PreservCyt Solution (Cytyc/Hologic, Marlborough, MA, USA) for H-HPV DNA testing.
Sampling locations were obtained using a hand held GPS unit (Garmin, Olathe, KS, USA).
For doing so, sampling frames were obtained for each kebele by taking the list of all household heads from the kebele offices.
Sampling permits were obtained from relevant authorities.
Analysis results from all sampling points were obtained from 77 participants.
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