Minimum evolution phylogeny with bootstrapping (500 re-samplings) was performed using MEGA 3.1 [ 34].
Manual sampling was performed using a 30 mm sampling spear with eight chutes.
The sampling step was performed using a PDMS-DVB fiber and desorption was carried out in a novel homemade heated interface.
The sampling process was performed using free publicly available GIS data sources and enables further linkage to specific obesity-related behavioural characteristics of environments, for example, using free publicly available remote imaging.
The rigid body docking of Mot1NTD TBP DNA NC2 crystal structure was performed using colores applying a 10° sampling step size (Chacón and Wriggers, 2002).
The global step was performed using Simulated Annealing with a modified sampling step, where we used a kernel of truncated normal distributions in order to include boundaries for the parameters (all parameters were assumed to lie within [1e-8, 1]) [36].
The shear test was performed using servo hydraulic system and recorded data acquisition system with sampling rate of 10 Hz for all experiments.
Logistic regression was performed using the ROBUST command in STATA to adjust for our sampling scheme and clustering of cases within households.
Imaging was performed using a Zeiss LSM 510 META inverted confocal microscope (Carl Zeiss, Germany) using a previously developed rapid sampling approach building on the mitotic history of sibling cells [ 9].
Secondly, a Bayesian Inference (BI) analysis was performed using MrBayes 3.1.2 [ 52], simulating four simultaneous Markov chains (MCMC) for 4 × 106 generations each, and using a sampling frequency of 100 generations.
Sampling calculations were performed using the Win Episcope 2.0 software http://www.clive.ed.ac.uk/winepiscope.ac.uk/winepiscope
