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Animals found infected at each sampling were treated with diminazene aceturate at 7 mg kg−1 body weight.
Paper points used for sampling were treated with 360 μl lysozyme solution for 30 min at 37°C (20 mg/ml lysozyme, 20 mM TrisHCl, 2 mM EDTA, 1.2% Triton X100, pH 8.00), followed by proteinase K digestion for 30 min at 56°C in 400 μl buffer AL (Qiagen, Hilden, Germany).
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Bioreactor and foamate samples were treated equally.
All samples were treated for 1 min.
The samples were treated with HPPIB.
Apple-juice samples were treated with pectinase and amylase.
RNA samples were treated with DNAse to remove DNA contamination.
All samples were treated by a solid phase extraction step.
Samples were treated with and without Ca addition.
Also, the control samples were treated with 1 ml ethanol.
Afterwards, the samples were treated by a standard RCA process.
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