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The hydrocarbon contents from each sampling were subjected to extraction with dichloromethane every 10 (aerobic consortia) or 30 days (anaerobic and mixed consortium).
Additionally, the data for the last sampling were subjected to Categorical Principal Components Analysis (CATPCA) using the statistical analysis program SPSS, version 18, to determine the association between all the variables.
The present study comprise 10 of the colic and 10 of the healthy horses that, in addition to plasma and muscle biopsy sampling, were subjected to muscle microdialysis.
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Samples were subjected to 3 freeze-thaw cycles.
The samples were subjected to ELISA for IL-2 concentration.
Treated samples were subjected to XRD analyses.
The samples were subjected to heat treatment.
Beads were removed and samples were subjected to PAGE on Amersham ECL Gel 4 20% (GE Healthcare).
Muscle tissues were collected, and blinded samples were subjected to the following procedure.
Samples were subjected to SDS-PAGE and transferred to nitrocellulose or PVDF membranes.
Twenty micrograms per sample were subjected to SDS PAGE and transferred to Immobilon P-membrane (Millipore).
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