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For each experimental time-point following bioreactor inoculation, the supernatants acquired from sampling were filtered and analytes measured using a Bioprofile 400 Analyser (Nova Biomedical, Waltham, MA).
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Then the samples were filtered, concentrated.
All the samples were filtered before analysis.
Samples were filtered through Whatman No. 2 filter paper.
All the samples were filtered through 0.45 mm membrane filter.
The samples were filtered through 0.45-μm Millipore membrane filters.
All samples were filtered through 0.22 μm polycarbonate membrane filter.
Samples were filtered through 0.2 μ m filters.
The concentrated samples were filtered through 0.45 μm membrane filters.
Samples were filtered through 0.45 μm membrane filter.
The samples were filtered by micro syringe filters (0.2 µm).
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