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The concatenated data set and three sub-samples with a different species sampling were analyzed with two different probabilistic methods, i.e. Maximum Likelihood (ML) as implemented in the program RAxML [ 62] and Bayesian Inference with PhyloBayes [ 64].
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Blood samples were analyzed with a blood gas analyzer (ABL90 Flex, Radiometer, Neuilly-Plaisance, France).
TOC of samples were analyzed with a multi N/C 2000 Analytic Jena analyzer.
Samples were analyzed with a Zeiss LSM710 Confocal Laser Scanning Microscope (Carl Zeiss, Oberkochen, Germany).
Then, samples were analyzed with a JSM-6700F electron microscope (Jeol) using secondary electrons at 5 kV.
Samples were analyzed with an Accuri C6 flow cytometry instrument and the data were processed using Flowjo v10 software.
Arterial plasma samples were analyzed with ELISA prior to and ~20 min following bradykinin stimulation (Fig. 4h, Supplementary Fig. 5).
Significant differences between samples were analyzed with separate ANOVAs followed by post-hoc tests, as indicated in the results Section of each experiment.
To qualitatively determine chemical composition, samples were analyzed with energy dispersive x-ray spectroscopy (EDXS) using an FEI Quanta FEG 450 FESEM (Hillsboro, OR).
Main Outcome Measures: Arterial blood samples were analyzed with a radiometer to measure PaO2 and PaCO2.
Samples were analyzed with a FACScan flow cytometer (Becton Dickinson, Mountain View, CA, USA).
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