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All sampling was conducted as randomly as possible with full geographical dispersal within each of the countries.
Tissue sampling was conducted as previously described [25].
The sample of patients is described in table 3. The sampling was conducted as a theoretical sampling in accordance with GTM [ 29], i.e., data were collected continuously and in interaction with data analyses.
A nationwide survey of male and female adolescent behavior using two-stage clustered sampling was conducted as part of the Youth Profile of the Republic of Mauritius in 1996.
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Shin et al. (2012) demonstrated similar distribution of TDS but the authors attributed the low TDS concentration at upstream to surface water runoff and eliminated the possibility of ocean influence as their sampling was conducted at low tide.
Virologic sampling is conducted as described above.
In the locations identified in Figure 1, mist netting and sampling were conducted as described (11 ).
Statistical analysis to calculate the standard deviations for relative expression in different samples was conducted as described in the Applied Biosystems Taqman protocol handbook.
The macrophage count in the ESCC tissue samples was conducted as described previously.
The binding assay for insect protein samples was conducted as described [ 5].
Genotyping of T. whipplei detected in the BAL fluid samples was conducted as described previously (24 ).
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