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In the second stage of sampling, village leaders led field teams to the village centre where a pen was spun to determine the field team's walking direction.
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The three sampling villages included in the study: Bozanta Mare (BM), Sasar (SA), and Recea (RC).
Census sampling targeting the entire livestock population of the two sampling sites was performed in July (Funyula site) and October (Butula site) 2004, by visiting all livestock keeping homesteads in all 19 sampling villages.
In the first stage of sampling, villages were enumerated and selected randomly with probabilities proportional to each village's population.
Annual incidence of NTD from 1998 to 2005 was recorded for each of the 326 sampling villages within Heshun County.
Samoan operator Green Turtle Holidays runs guided tours to sample village life on the islands, including courses on reef fishing and traditional medicine.
Ten investigators with prior training4 each conducted household questionnaire surveys in 2 4 households per sample village.
Ten investigators with professional training background each conducted household questionnaire surveys in 2 4 households per sample village.
The average number of goats in each sampled village household was about 5. The average herd size per household in different districts including the study area is shown in Table 1.
In contrast to the overall high NRY haplogroup diversity for the entire sample, village based pockets of low diversity were observed, consistent with local paternal founder effects.
Then, households within each sample village were selected through systematic random sampling.
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