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Each respondent was asked to extract water from the storage container as s/he normally would and to pour it into the sampling vessel.
The Salivette sampling device with no preservative (Sarstedt) was used, the tube consisting of a plastic sampling vessel with a sterile neutral cotton wool swab, which had to be chewed for about 30 s and then returned to the insert.
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Sampling vessels (polypropylene, 50 mL; Greiner Bio-One, Frickenhausen, Germany) were thoroughly rinsed with methanol before use.
Before the sampling process, approximately 15 l of the fermenter substrate were discarded before aliquots of 1 l were transferred into clean, gastight sampling vessels and transported directly to the laboratory.
A high-pressure steel tubing connected the sample vessel to the gas meter.
The impurity enrichment factors for such sampling devices are functions of the sampler size, and the sample vessel pressures before and after enrichment.
The non-uniform intensities in the given image are estimated and corrected properly.A sample vessel segmentation result for a non-pathological fundus image from our dataset is shown in Figure 16.
Also, the image could not be segmented employing the proposed segmentation method without preprocessing as depicted in Figure 16g.Another sample vessel segmentation result for a non-pathological fundus image from STARE dataset is depicted in Figure 17.
The step velocity of the tetragonal hen egg-white lysozyme (1 1 0) crystal plane toward the [0 0 1] direction could be controlled and measured accurately on a nanometer scale using a specially designed sealed sample vessel on an atomic force microscope.
Then 10 mL of HNO3 was added to the sample vessel.
Two ml lysate was aliquoted into a NucliSENS easyMAG sample vessel and eluted in 25 μL of elution buffer.
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