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Genomewide studies of large datasets are showing that, for reasons not clear yet, but probably due to sampling, technical or genotyping differences, the top signals are not consistently replicating in independent samples (the winner's curse).
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Multimammate rats were the dominant commensals at all sampling locations, comprising 64.5% of the overall sample (Technical Appendix Figure).
This reaction was performed in triplicate for each sample (technical replicates).
Data reliability was assessed by checking Pearson correlation coefficient C among samples: technical replicates resulted more correlated among themselves (C>0.96).
Amplification was positive in 19/85 (22.4%) samples; sequences were obtained for 18 of these samples (Technical Appendix).
For 3 of the samples, technical replicates of the capture and sequencing were performed.
Borrelia spp. DNA was detected in 5 (36%) of 14 serum samples (Technical Appendix Table).
For each cDNA sample, technical duplicates in each of three biological replicates were tested.
B. pilosicoli was the most common species, found in all 10 samples (Technical Appendix, panel I).
Although CISH demonstrates specificity in these samples, technical problems may influence sensibility when compared with FISH.
SWAN is a within-sample normalization procedure, however it has been proposed for reduction of between-samples technical variability.
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