Specimens collected on one sampling day in the three traps were pooled per sampling station, resulting in a total of 216 butterfly samples (24 sampling stations ×9 days).
Each sampling station was defined by laying out a 3-foot-diameter sampling ring, and three equally spaced cores were collected within the ring.
Five branch portions (5 cm) were cut from five parent colonies at each sampling station (~5 m depth) and frozen until analysis.
Direct gradient analysis showed that the proportion of vegetated area within a 50 m radius of the sampling station explained 15% of the variation in bacterial community composition.
These were then applied to the temporal series obtained and the one that best reflected the annual bioconcentration at a sampling station was selected.
The principal component analysis of sediment data (32 samples × 9 metals) indicated different patterns of contamination regarding the tributary and sampling station.
To determine whether the erosion of diversity closer to edges was due to altered plant-enemy interactions, we applied fungicide and insecticide treatments to a subset of plots at each sampling station (Fig. 1d).
When the crystalline fraction for rocks, rakes and 4 10 rum fragments is compared for each sampling station, the relative abundance of crystalline material is consistent in all sizes, but differences between sampling stations within a landing site are distinct.
A single sampling station is a time interval and region of space that should be small compared to the respective time and space scales of variation of the phenomenon being studied.
Both multibeam backscatter data and QTC VIEW™ number-coded classifications were extracted across a range of circular areas located at each georeferenced sampling station (radii: 2, 3, 4, 5, 8, 12, 16, 20 m).
A sediment weight of about 30 g each was collected from each sampling station.
