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In this study, sampling of sera after 31 weeks from only first and boosting vaccinated cattle was limited, due to national FMD control policy by which re-booster vaccination carried out every 6 months in cattle.
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For validating this assay, 140 clinical samples of sera were used, comprising 100 samples from patients who tested positive for anti-HDV and hepatitis B virus surface antigen (HBsAg) by ELISA; 30 samples from blood donors; 5 samples monoinfected with hepatitis B virus (HBV); and 5 samples monoinfected with hepatitis C virus (HCV).
Serially diluted (2-fold increments) samples of sera and BAL fluid, as indicated in the figures, were used to derive the antibody titration curves.
Field samples of sera from bovine TB or MAP infected animals were obtained from serum repositories at National Veterinary Services Laboratory or from routine sample acquisitions into Minnesota Veterinary Diagnostic Laboratory, respectively.
DNA obtained from twenty-five celineagesges (ten from cultures in routine multiplication and fifteen from frozen ampoules), nine samples of sera used in cell culture media and five batches of trypsin were examined for the presence of TTSuV DNA.
The initial sera were re-tested simultaneously with the second samples of sera.
Twenty-eight random samples of sera were taken from each group of subjects for analysis.
Besides this, a random sample of sera of beef animals were tested since 1993 (see Table 2).
All samples of sera were stored in the −80 °C degree freezer until ELISA was performed.
Two samples of sera were available for 48 cases of non-Hodgkin's lymphoma and their matched controls.
Of the 118 case control pairs with two samples of sera, for 116 there was sufficient volume of sera available to test for lytic anti-KSHV antibodies.
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