Samples of heat inactivated (95 °C, 10 min) enzyme served as negative controls.
A sample of Heat was distributed alongside each copy of Beyoncé's live album, I Am... World Tour (2010) and her fourth studio album, 4 (2011).
Ten µl of the 100× bacteria suspension were diluted further in PBS and were used to inoculate each 250 µl sample of heat inactivated (95°C, 5 min) AF, resulting in a final average inoculum of ∼104 CFU/ml.
The interface between plates and spacer is too much smaller relative to the area of plates so that the effect of materials properties of the samples on heat conduction of air can be ignored.
The dark cultivation lasted up to 105 h, during which samples of heat-treated and untreated cells were respectively taken for analysis at various time points as indicated below.
Consequently, nominal differences were observed between the samples of heat-inactivated bacteria and sterile medium (Figure S5).
Sample-to-sample heat map analysis of RNA-Seq data showed a uniform expression pattern among the biological replicates (Additional file 7: Figure S2).
The result: Bridge pays a yearly $250,000 licensing fee to carry samples of his heat maps, while its sales force is out flogging the full product line for him, taking a 25% cut of revenues.
A representative sample of the heat map of the global changes in the transcript levels of genes during freezing and the post -freezing recovery period is given in Additional file 1: Figure S4.
Hardness of samples as function of heat treatment was also measured using Janka hardness (ASTM D 1037 12).
