For female mice, duplicate samples of embryonic ovaries were collected at 11.5, 12.5, 14.5, 16.5, and 18.5 days of postcoitum [ 9].
To further investigate the evolution of the Nups network, we divided the sample of embryonic genes into two groups: The Nups network and the rest of embryonic genes and compared their rate of evolution in each species.
Developmental and morphological studies have been largely performed on human choroid plexus samples of either embryonic or post-mortem origin.
In cultures of seminoma samples, the expression of embryonic markers (PLAP) was retained, and the morphology of seminoma samples was relatively well preserved up to 7 days in hanging drop cultures.
Sample collection was performed consistently to avoid contamination of embryonic samples with maternal membranes, as this would have jeopardized correct determination of chromosomal inheritance.
Zebrafish samples for qPCR consisted of embryonic and adult hearts and trunk muscle, and were isolated as described above.
To gain insight into MTP expression by the avian embryo, we prepared liver, kidney and small intestine samples from different stages of embryonic development (10, 15 and 20 days), and from 1 and 3 day old chicks, respectively.
The recent application of single-cell RNA-Seq has allowed profiling of samples with limited quantities of sample such as embryonic development, but this technique did not discriminate strand-specific transcripts and did not detect 5' ends of transcripts longer than 3 kb which would hinder analysis of alternative promoter usage [ 16, 17].
This indicates that our samples represent the native development of embryonic towards adult epicardium [18].
The use of embryonic tissue samples were approved by Ethics Committee of the University of British Columbia, Faculty of Medicine.
After removal of embryonic tissues, samples were finely minced and digested with collagenase type II (1 mg/mL), collagenase type IV (2 mg/mL) and dispase II (0.02 mg/mL) for 1 h at 37°C.
